In OVX mares and one intact mare, positive ERalpha staining was also detected in adrenal medullary cells. Ovariectomy increased cortical ERalpha staining intensity. ResultsĮRalpha staining was detected in the adrenal zona glomerulosa, fasciculata and reticularis of all mare groups. Uterine tissue was used as a positive control and showed positive staining for both ERalpha and PR. Three of the OVX mares were treated with a single intramuscular injection of oestradiol benzoate (2.5 mg) 18 – 22 hours prior to euthanasia and tissue collection (OVX+Oe). Adrenal glands from intact (n = 5) and ovariectomised (OVX) (n = 5) mares, as well as uterine tissue (n = 9), were collected after euthanasia. In the present study, immunohistochemistry using two different mouse monoclonal antibodies was employed to determine the presence of oestrogen receptor alpha (ERalpha) and progesterone receptor (PR) in the mare adrenal gland. Sex steroid hormone receptors have been identified in the adrenal gland of rat, sheep and rhesus monkey, indicating a direct effect of sex steroids on adrenal gland function.
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